Signalling

Part:BBa_K511401:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


HRH4-TEVs-GV16 Signaling Receptor MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 551
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 551
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 551
    Illegal BglII site found at 713
    Illegal BamHI site found at 1178
    Illegal BamHI site found at 1208
    Illegal XhoI site found at 1674
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 551
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1593
    Illegal SapI site found at 863


Design Notes

The TEV recognition sequence encoded in the rough center of this part is flanked on both sides by sequences that code for glycine-serine-glycine linkers. This feature was designed to serve as a homologous region allowing for facile substitution of either a receptor or a transactivator around the TEV recognition sequence using the Gibson ligation assembly method.


Source

This part includes human histamine receptor 4 (HRH4), the tobacco etch virus (TEV) protease recognition sequence, the DNA-binding domain from the Gal4 transactivator of Saccharomyces cerevisiae, and the VP16 transactivation domain from the herpes simplex virus.


References